Rat Caspase-7 ELISA Kit

From Assay Genie, this Sandwich ELISA (double antibody) kit is designed for the quantitative measurement of Caspase-7 in rat samples. Reactivity is rat, and validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is intended for research into apoptosis and cell death pathways, where Caspase-7 acts as an execution-phase enzyme whose dysregulation has been implicated in cancer, neurodegenerative disorders, and autoimmune diseases.

Reported performance figures from the manufacturer include a sensitivity of 0.188 ng/mL and an assay range of 0.313-20 ng/mL, with absorbance read at 450 nm. Reagents are stored at 2-8°C for 12 months. Spike-and-recovery in serum (n=5) is 83-101% (average 95%), in EDTA plasma (n=5) 89-104% (average 96%), and in heparin plasma (n=5) 90-99% (average 94%). Linearity across 1:2, 1:4 and 1:8 dilutions is 86-96% / 86-96% / 86-104% for serum (n=5), 85-100% / 83-98% / 84-99% for EDTA plasma (n=5), and 88-96% / 81-98% / 82-97% for heparin plasma (n=5).

The kit is supplied in a solid-phase format and has been cited in independent work, including Chen et al. (Cellular and Molecular Neurobiology, 2021; PMID: 32715402), who used it to study apoptosis and inflammation in a rat spinal cord injury model via the mTOR signalling pathway.