Rat Carboxylesterase 1 (CES1) ELISA Kit

Quantitative measurement of hepatic carboxylesterase 1 (CES1) supports toxicology, pharmacology, and drug metabolism research in rodent models. The Assay Genie Rat Carboxylesterase 1 (CES1) ELISA Kit provides an in vitro immunoassay for detecting CES1 in rat-derived samples, enabling studies of xenobiotic hydrolysis, prodrug activation, and lipid processing.

Carboxylesterase 1 is a serine hydrolase that cleaves ester and amide bonds in substrates ranging from narcotics and ester prodrugs to triglyceride-derived lipids. The enzyme is abundantly expressed in rat liver, making the rat a key species for modeling hepatic clearance pathways relevant to human pharmacology. Investigators apply this kit in protocols for hepatotoxicity screening, liver injury biomarker assessment, diet-induced obesity models, type 2 diabetes research, and pesticide exposure evaluations. Compatible sample matrices include rat liver tissue homogenates prepared from fresh or frozen specimens.

Each kit is supplied in a microplate configuration, with capture antibodies, detection reagents, standards, and buffers included. Samples, standards, and controls are added to the plate, and absorbance is recorded using a standard plate reader. Reagents should be stored refrigerated and handled according to the included protocol to preserve activity across the stated shelf life.