Rat Calsequestrin-1/CASQ1 ELISA Kit

Manufactured by Assay Genie, this colourimetric Sandwich ELISA using a double-antibody format is designed for the accurate quantitative measurement of Calsequestrin-1 (CASQ1) in rat-derived samples. CASQ1 is a key protein involved in calcium storage and release in muscle cells and is critical for proper muscle contraction and relaxation. Dysregulation of CASQ1 has been implicated in muscle disorders including malignant hyperthermia and central core disease, making the kit a useful tool for researchers studying muscle physiology, muscle-related disease and potential therapeutic strategies.

The assay is reactive with rat samples and is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported sensitivity is 46.875 pg/mL with a standard curve range of 78.125–5000 pg/mL. The kit is intended to be stored at 2–8°C for 12 months. Synonyms for the target recognised by the antibody pair include Casq1, Calsequestrin-1, Laminin-binding protein, Aspartactin, and Calsequestrin skeletal muscle isoform (UniProt P19633).

Manufacturer-reported linearity for serum (n = 5) spans 92–100% at 1:2, 88–104% at 1:4 and 89–105% at 1:8; EDTA plasma (n = 5) shows 86–99%, 82–90% and 87–100%; heparin plasma (n = 5) shows 84–100%, 87–101% and 80–104%. Recovery ranges across the same matrices are 85–103% (average 97%) for serum, 95–107% (average 94%) for EDTA plasma and 82–102% (average 94%) for heparin plasma.