Rat Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ELISA Kit

Quantitative immunoassay for rat calcium/calmodulin-dependent protein kinase II gamma from Assay Genie, formatted for 96 assays per kit and applied to tissue homogenates and cell lysates derived from rat nervous system and peripheral tissues where the gamma isoform is endogenously expressed. CaMKII gamma participates in calcium-triggered signal transduction that controls synaptic plasticity, neurotransmitter release, and excitation-transcription coupling, and isoform-specific detection matters when researchers need to separate the gamma subunit from the more abundant alpha and beta CAMK2 subunits that often coexist in the same lysate.

The workflow uses a biotinylated detection antibody with streptavidin-enzyme conjugate, a TMB chromogenic substrate, and a stop reagent producing a stable endpoint signal read on a standard microplate spectrophotometer. Each kit ships with a recombinant rat CAMK2g calibrator for building a standard curve, biotinylated detection reagent, wash concentrate, assay buffer, substrate solution, and plate seals. Reagents are packaged in single-use vials to limit repeated freeze-thaw of working stocks, and reconstituted components are kept refrigerated between runs.

Applications include studies of cerebellar Purkinje cell signaling, hypothalamic and pituitary regulation, cardiac calcium handling, and smooth muscle contractility where CAMK2g abundance shifts under experimental or disease conditions. Researchers comparing knockout versus wild-type rats, or profiling kinase expression across brain regions and peripheral organs, can run the full 96 assays in a single batch with the included calibrator and buffer set.