Rat Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) ELISA Kit

CELSR2 belongs to the flamingo subfamily of non-classical cadherins within the cadherin superfamily. These atypical cadherin proteins participate in planar cell polarity signaling, contact-mediated communication, and neural tube closure during embryonic development. In adult rat tissue, CELSR2 expression has been mapped to vascular endothelium and selected neuronal populations.

The Rat CELSR2 ELISA Kit from Assay Genie provides a quantitative immunoassay for measuring CELSR2 protein in rat-derived samples. Researchers studying cardiovascular biology, neurodevelopment, and planar cell polarity use this approach to track CELSR2 abundance across experimental cohorts. The kit is supplied in a 96-assay microplate format with colorimetric readout, and is validated for rat tissue homogenates.

Working with rat tissue homogenates requires careful sample preparation: homogenize fresh or flash-frozen tissue in a chilled lysis buffer with protease inhibitors, clarify the lysate by centrifugation, and aliquot the supernatant before storage. Avoid repeated freeze-thaw cycles, since CELSR2 epitope integrity is best preserved when aliquots are kept frozen and protected from light until the assay run.

Typical applications include gene knockout validation, time-course studies of CELSR2 expression in disease models, and comparative quantification between treatment groups in vascular and neurological research programs. The kit is intended for research use only and is not validated for diagnostic procedures.