Rat C1 (Complement Component 1) ELISA Kit (RTES00919)

Complement Component 1 (C1) is the initiating trigger of the classical complement pathway, a large macromolecular complex composed of C1q, C1r, and C1s subunits that recognizes antibody-antigen aggregates bound to immunoglobulins on target surfaces. Using this assay from Assay Genie, researchers typically run serial dilutions of unknown samples alongside the calibrated reference standards to interpolate concentrations from a fitted standard curve. The enzymatic substrate reaction produces a measurable absorbance change read with a standard plate reader, giving reproducible quantification across biological replicates. Independent verification of rat C1 levels in vivo supports studies of glomerulonephritis, ischemia-reperfusion injury, serum sickness, and antibody-mediated rejection, where classical-pathway activation drives downstream C3 and C4 cleavage. Each reagent vial is supplied ready to use and should be stored refrigerated and protected from light per the included handling insert. For best results, avoid repeated freeze-thaw cycles of the reconstituted standards and aliquot working volumes before long-term storage at -20 degrees C when extended study timelines apply. The assay is intended for research use only and is not validated for clinical diagnostic workflows.