Rat C-Type Natriuretic Peptide (CNP) ELISA Kit

Quantitative enzyme immunoassay configured to quantify endogenous CNP-22 in rat specimens, supporting cardiovascular physiology, endocrinology, and skeletal biology investigations. The microplate is supplied pre-coated with anti-CNP capture antibody and packaged with a biotinylated detection reagent, streptavidin-HRP conjugate, and TMB substrate solution, generating an absorbance readout proportional to peptide concentration in the well.

CNP is the principal natriuretic peptide of the central nervous system and is also released by vascular endothelial cells and growth-plate chondrocytes, where it signals through the NPR-B receptor to drive smooth muscle relaxation, axon pathfinding, and endochondral ossification. Researchers working with rat disease models rely on a CNP detection kit to track circulating peptide concentrations, tissue homogenate levels, or conditioned media accumulation in response to pharmacological or genetic interventions.

Each pack contains a microplate and ancillary reagents sufficient for 96 reactions. Sample types validated for the workflow include rat serum, EDTA plasma, and heparin plasma; rapid cold processing after collection is recommended because endogenous CNP is cleared quickly in vivo. The provided protocol walks through standard curve generation, recommended sample dilutions, and the wash and incubation schedule used in research use only applications.

Assay Genie reports specificity testing showing that the capture and detection antibodies raised against rat CNP-22 give minimal recognition of the related natriuretic peptides ANP and BNP, an important consideration for studies in which these vasoactive hormones co-circulate. Reagents should be stored refrigerated at 4 degrees C upon arrival to preserve binding activity through the stated shelf life.