Rat C-JUN ELISA Kit

Designed for the precise measurement of c-jun levels in rat samples, this Sandwich ELISA Kit from Assay Genie offers high sensitivity and specificity for research applications. C-Jun is a key transcription factor involved in cell growth, differentiation, and apoptosis, and its dysregulation has been linked to cancer, inflammatory disorders, and neurodegenerative conditions.

The kit works with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, using a colorimetric sandwich ELISA, double antibody format. Reported performance figures include a sensitivity of 0.938 ng/mL and an assay range of 1.563–100 ng/mL. The target protein is referenced under UniProt accession Q9R237, and kit components should be stored at 2-8°C for up to 12 months. The procedure uses a biotin-labeled detection antibody, HRP-Streptavidin conjugate, TMB substrate, and required buffers, with absorbance measured at 450 nm after the reaction is stopped.

Validation data from the manufacturer covers linearity and recovery across serum, EDTA plasma, and heparin plasma. Linearity at 1:2, 1:4, and 1:8 dilutions is 88-102% / 87-104% / 87-104% in serum, 82-96% / 82-91% / 87-99% in EDTA plasma, and 87-102% / 82-94% / 81-99% in heparin plasma. Average recovery values are 92% for serum, 94% for EDTA plasma, and 95% for heparin plasma, with corresponding ranges of 86-102%, 86-103%, and 91-103% respectively.