Rat BMP2 ELISA Kit

Manufactured by Assay Genie, this colorimetric Sandwich ELISA kit uses a double-antibody method to quantitatively detect Rat Bone Morphogenetic Protein 2 (BMP-2), also known as BMP2A or BMP-2A. The kit is validated for use with rat serum, plasma, and cell culture supernatants, and is intended for research use only. BMP2 is a member of the TGF-beta family that induces cartilage and bone formation, and has implications in research on bone disorders, osteoporosis, and tissue regeneration.

Performance specifications include a sensitivity of 37.5pg/ml and an assay range of 62.5-4000pg/ml. Spike-and-recovery testing yielded average recovery rates of 96% for serum (range 88-102%), 99% for EDTA plasma (range 90-104%), and 98% for UFH plasma (range 89-103%). Linearity across 1:2, 1:4, and 1:8 dilutions spanned 82-105% across the three matrices. Precision was demonstrated with an intra-assay CV <8% and an inter-assay CV <10%. The kit is stored at 4°C for 6 months.

The target protein is catalogued under UniProt P49001 (NCBI Gene ID 29373) with a molecular weight of 44,383 Da. BMP2 is a secreted signaling protein implicated in bone mineralization, osteoblast differentiation, chondrocyte differentiation, cartilage development, ossification, heart development, and the BMP signaling pathway.