Rat beta-NGF PharmaGenie ELISA Kit (For Lysates) (SBRS1612)

This Sandwich ELISA from Assay Genie enables quantitative measurement of the mature beta subunit of nerve growth factor in rat cell and tissue lysates. Beta-NGF is a neurotrophin central to studies of peripheral nerve injury, neuropathic pain, sympathetic neuron survival, and neuroinflammation. The pre-coated microplate format accommodates homogenized tissue samples from brain, sciatic nerve, dorsal root ganglia, or cultured neuronal lines.

Performance specifications:

• Standard detection range: 15.6 to 1000 pg/mL
• Sensitivity: less than 5 pg/mL
• Sample volume: 100 uL per well
• Total assay incubation: approximately 4.5 hours at room temperature
• Storage: refrigerate at 2 to 8 degrees C; protect reagents from light
• Species reactivity: rat; significant cross-reactivity with other species has not been observed

The kit includes a capture antibody specific to rat beta-NGF, a biotinylated detection antibody, streptavidin-HRP conjugate, recombinant rat beta-NGF standard, and all buffers required for lysate preparation. Researchers studying neurotrophin signaling, inflammatory pain pathways, or neuronal differentiation can use this assay to compare beta-NGF levels across experimental conditions. For research use only; not intended for diagnostic or therapeutic applications.