Rat beta MSH/beta-Melanocyte Stimulating Hormone ELISA Kit

Designed for the accurate measurement of Beta-MSH (Beta-Melanocyte Stimulating Hormone) in rat samples, this colorimetric Sandwich ELISA from Assay Genie provides a sensitive, double-antibody method for quantifying the peptide across serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Beta-MSH is described as a key peptide hormone involved in regulating pigmentation, energy balance, and immune function, with dysregulation linked to metabolic disorders, inflammatory diseases, and skin conditions, making it a useful biomarker in rodent research models.

The assay reports a sensitivity of 46.875 pg/mL and a measuring range of 78.125-5000 pg/mL, with detection via TMB substrate and absorbance read at 450 nm. The workflow involves equilibration of reagents, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding at 37°C for 60 minutes, HRP-streptavidin (SABC) binding at 37°C for 30 minutes, and color development in the dark for 10–20 minutes before stop and reading. Reagents are stored at 2-8°C for 12 months.

Validation data from the manufacturer show linearity across dilutions: serum (n=5) 86-103% at 1:2, 86-99% at 1:4, 86-98% at 1:8; EDTA plasma (n=5) 92-98%, 90-101%, 83-100%; heparin plasma (n=5) 81-100%, 80-99%, 86-98%. Spike-and-recovery performance averaged 98% for serum (range 87-105%), 96% for EDTA plasma (range 85-103%), and 100% for heparin plasma (range 94-105%).