Rat BECN1 (Beclin 1) ELISA Kit  (RTES00927)

Autophagy regulation in rat tissues can be quantified at the protein level with this ELISA Kit from Assay Genie, which targets Beclin 1, the ~52 kDa scaffold of the class III phosphatidylinositol 3-kinase complex. Beclin 1 governs autophagosome nucleation and drives autophagic flux in mammalian cells, making it a widely studied marker of cellular self-digestion.

Autophagy research in rat models frequently monitors Beclin 1 because its abundance shifts in response to nutrient stress, hypoxic injury, neurodegenerative challenges, and tumor microenvironment cues. The kit supports studies of cardiac ischemia-reperfusion injury, hepatic steatosis, neuroinflammation, and chemotherapy response in rodent workflows, where it is paired with western blot and immunofluorescence readouts to triangulate protein-level changes.

Each kit contains a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, calibrated standards, and assay buffer sufficient for 96 determinations. The standard workflow follows ELISA protocols with the supplied reagents, allowing researchers to generate standard curves and interpolate Beclin 1 concentrations from prepared liquid samples and tissue lysates. Storage of unopened kits at refrigerated temperatures preserves antibody activity and standard stability across freeze-thaw cycles.

By providing a quantitative readout of Beclin 1 protein abundance, the kit complements Becn1 transcript analyses and supports longitudinal monitoring of autophagy engagement in vivo across experimental cohorts.