Rat BAD/Bcl2 antagonist of cell death ELISA Kit

The Assay Genie Rat BAD ELISA Kit is a sandwich, double-antibody immunoassay designed for the quantitative detection of BAD (Bcl2 antagonist of cell death) in rat samples. The kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and uses a colorimetric detection method with absorbance measured at 450 nm.

Stated performance includes a sensitivity of 18.75 pg/mL and a working range of 31.25-2000 pg/mL, with reagents stored at 2-8°C for 12 months. The target (UniProt: O35147) is described as a key regulator of cell death that plays a critical role in apoptosis and cell survival pathways, supporting research applications across cancer, autoimmune disorders, and neurodegenerative conditions.

The procedure includes a 90-minute primary incubation at 37°C, a 60-minute biotinylated detection antibody step, a 30-minute HRP-Streptavidin (SABC) incubation, followed by 10–20 minutes of TMB substrate development in the dark before the reaction is stopped and absorbance is read. Reported linearity and recovery data are provided for serum, EDTA plasma, and heparin plasma across serial dilutions.