Rat Atrial natriuretic peptide receptor 2/NPR2 ELISA Kit

Assay Genie's Rat Atrial natriuretic peptide receptor 2 (NPR2) ELISA Kit is a sandwich ELISA, double antibody assay designed for the precise measurement of NPR2 levels in rat samples. NPR2 is a key receptor involved in the regulation of natriuresis and blood pressure, with dysregulation implicated in various cardiovascular diseases, making it a valuable biomarker for cardiovascular research.

The assay demonstrates a sensitivity of 46.875 pg/mL and a detection range of 78.125-5000 pg/mL, using a colorimetric detection method. It is compatible with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with kit components stable at 2-8°C for 12 months. The protocol involves sequential incubation steps with biotin-labeled detection antibody, HRP-Streptavidin (SABC), and TMB substrate, with absorbance read at 450 nm.

Validated performance includes serum linearity ranges of 90-103%, 91-104% and 85-100% at 1:2, 1:4 and 1:8 dilutions, and recovery rates averaging 96% for serum, 93% for EDTA plasma, and 99% for heparin plasma.