Rat ATPase, Na+/K+ Transporting Beta 3 Polypeptide (ATP1b3) ELISA Kit

ATP1b3 encodes the beta-3 subunit of the Na+/K+ ATPase, a transmembrane P-type ATPase that maintains electrochemical gradients across cell membranes by exchanging intracellular sodium for extracellular potassium. Quantification of ATP1b3 protein in rat models supports work on neuronal excitability, cardiac contractility, and renal tubular ion handling, where changes in subunit composition alter pump kinetics and ouabain sensitivity.

This Assay Genie ELISA Kit is configured for rat samples and accepts tissue homogenates prepared from organs of interest. The microplate strip format supports up to 96 assays per run, with capture antibody pre-bound to wells, lyophilized standards, detection antibody, and substrate solution provided in sealed vials sufficient for the full plate. Signal is recorded on a standard absorbance plate reader, and results are interpreted against the recombinant ATP1b3 standard curve generated on each plate.

Pre-coated wells ship ready to use, and the complete workflow is suitable for in vitro research use only. Recommended sample handling includes rapid tissue collection, flash-freezing in liquid nitrogen, and homogenization in cold buffer with protease inhibitors to preserve epitopes. Researchers investigating sodium pump subunit remodeling, neurological disease models, or cardiovascular pathophysiology can run this kit alongside complementary assays for other Na+/K+ ATPase subunits such as ATP1b1 and ATP1b2.