Rat ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a) ELISA Kit

Quantitative immunoassay kit for measuring the catalytic alpha subunit of the gastric H+/K+ ATPase (ATP4a) in rat-derived specimens. The H+/K+ ATPase alpha polypeptide is the proton pump responsible for gastric acid secretion in parietal cells, and quantifying its expression supports research into acid-related disorders, peptic ulcer disease, Helicobacter pylori pathogenesis, gastroesophageal reflux, and pharmacological studies of proton pump inhibitors.

Manufactured by Assay Genie, the kit accommodates diverse sample matrices including tissue homogenate preparations, plasma-derived fractions, cell lysates, cell culture supernatant, and serum-based specimens, with the assay procedure run on a standard microplate reader. Each kit contains reagents for 96 tests in a single-use strip plate format. The absorbance-based readout enables straightforward quantification using routine laboratory instrumentation. Reagents should be refrigerated on arrival and handled according to the enclosed insert.

Researchers studying gastric physiology, parietal cell function, or screening compounds that modulate acid secretion can use this ELISA to track ATP4a levels across experimental conditions. Applications include gastric mucosa tissue analysis, primary parietal cell culture studies, and animal model investigation of acid secretion pathways.