Rat ATG5 ( autophagy protein 5) ELISA Kit (RTFI01413)

ATG5 (autophagy protein 5) is a core component of the autophagy machinery, forming a covalent conjugate with ATG12 that drives autophagosome membrane expansion and LC3 lipidation. Researchers use this rat-specific ATG5 ELISA Kit from Assay Genie to quantify endogenous ATG5 protein levels in serum and plasma, supporting investigations of nutrient stress responses, neurodegeneration models, tumor cell survival signaling, and mitophagy flux. The kit arrives as a complete microplate assay with pre-coated capture antibody, detection antibody, standards, and substrate. A 50 uL sample volume per well is sufficient for quantitative measurement, and standard curves are generated from seven calibrator points. Color development is stopped after a defined incubation period, and absorbance is read at 450 nm against a reference filter for accurate interpolation. No significant cross-reactivity with related ATG family proteins has been reported in the manufacturer's validation data. All kit components should be stored at 2-8 degrees C and equilibrated to room temperature before each run; this product is for research use only.