Rat Arginase (Arg) ELISA Kit

Quantitative immunoassay for measuring arginase (Arg) levels in rat-derived specimens, developed by Assay Genie for laboratories studying urea cycle metabolism, hepatic function, and macrophage polarization. The kit uses an antibody pair-based capture and detection format with absorbance readout on a standard plate reader, supporting studies of M1 versus M2 immune cell phenotypes, liver injury models, and arginine pathway regulation.

Validated across diverse sample matrices including serum-based collections, plasma-derived preparations, tissue homogenates, cell lysates, and cell culture supernatants, the assay enables flexibility across in vivo and in vitro experimental designs. Researchers apply it to wound healing investigations, hepatic disease models, cardiovascular research, and immunological studies where arginase activity or expression is a relevant biomarker. Each package contains reagents for 96 individual assays and is intended for research use only, not for diagnostic procedures.

Arginase is a manganese-dependent hydrolase enzyme that converts L-arginine to ornithine and urea, existing as two isoforms in mammals: ARG1 in the cytosol of hepatocytes and ARG2 in mitochondria of extrahepatic tissues. Dysregulated expression is implicated in liver fibrosis, asthma, parasitic infection, vascular dysfunction, and tumor immune evasion. Reagents should be stored refrigerated and protected from light to preserve antibody binding activity across the workflow.