Rat Arachidonate-5-Lipoxygenase (ALOX5) ELISA Kit

The Assay Genie rat ALOX5 ELISA kit quantifies arachidonate 5-lipoxygenase protein levels in laboratory rat samples, supporting research into leukotriene biosynthesis and inflammatory pathway signaling. ALOX5 is the dioxygenase that initiates conversion of arachidonic acid into 5-hydroperoxyeicosatetraenoic acid (5-HPETE) and downstream leukotriene mediators, making accurate protein quantification valuable in studies of airway inflammation, asthma models, and immune cell activation. The microplate format uses a colorimetric readout for batch processing of tissue homogenates and cell lysates. The kit accommodates multiple tissue homogenate preparations and cultured cell extracts, enabling researchers to monitor changes in ALOX5 expression under experimental conditions. Reagents are supplied for 96 assays, and the procedure fits standard laboratory incubation workflows. The assay targets rat ALOX5 specifically and is intended for rat-derived samples. Storage at refrigerated conditions preserves antibody binding activity across the shelf life. Arachidonate-5-lipoxygenase plays a central role in the 5-LOX pathway implicated in eosinophil and neutrophil responses, making this ELISA a useful tool for pharmacology, toxicology, and disease model investigations.