Rat Aquaporin 1, Colton Blood Group (AQP1) ELISA Kit

The Rat Aquaporin 1 ELISA kit from Assay Genie is a quantitative immunoassay for measuring AQP1 protein levels in rat-derived research samples. Aquaporin 1 (AQP1), also known as the Colton blood group antigen, is a membrane-bound water channel protein that plays a critical role in osmotic water transport across epithelial and endothelial tissues. In rat models, AQP1 is widely studied for renal concentrating ability, fluid homeostasis in red blood cells, choroid plexus cerebrospinal fluid secretion, and vascular permeability.

This 96-assay plate format delivers a complete reagent set including pre-coated wells, detection antibodies, standards, and substrate solution, allowing researchers to run full quantitative curves from tissue homogenates and liquid samples in a single workup. The assay uses a colorimetric readout measured on a standard microplate reader, making it accessible to laboratories without specialized instrumentation.

Applications include nephrology research on urinary concentration defects, studies of cerebral edema and stroke involving the blood-brain barrier, transfusion research on the Colton blood group system, and investigations of angiogenesis and tumor microenvironments where AQP1 expression has been implicated. Sample requirements are flexible, accommodating serum samples, plasma samples, tissue homogenates prepared from rat organs such as kidney, brain, or lung, and various fluid matrices.

The kit is supplied with a comprehensive protocol containing recommended dilution factors, incubation times, and standard curve generation guidance. Researchers studying water channel physiology, renal function, or comparative membrane biology will find this kit a targeted tool for quantifying AQP1 expression in rat experimental systems.