Rat Apoptotic Peptidase Activating Factor 1 (APAF1) ELISA Kit

APAF1 (Apoptotic Peptidase Activating Factor 1) is the central scaffold of the intrinsic apoptosis pathway. In response to cytochrome c release from damaged mitochondria, APAF1 assembles the heptameric apoptosome, which recruits and activates caspase-9 to initiate the caspase cascade driving programmed cell death. Dysregulated APAF1 expression has been implicated in tumor chemoresistance, neuronal loss after stroke, and cardiomyocyte apoptosis following ischemic injury, making it a widely studied biomarker across apoptosis-focused research programs.

This rat APAF1 ELISA Kit from Assay Genie is supplied as a complete reagent set in a 96-assay microplate format, compatible with standard laboratory plate readers. The kit includes pre-coated wells, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, and lyophilized protein standards. Each vial is intended for single-use setup, and storage temperature recommendations should be observed to preserve antibody binding activity. Researchers typically load 50 uL of tissue homogenate or cell lysate per well, with parallel standard curves for absolute quantification.