Rat Apolipoprotein A1 (APOA1) ELISA Kit

Developed by Assay Genie, this rat-specific Apolipoprotein A1 kit is built on a sandwich ELISA architecture and is widely used in cardiovascular and metabolic disease research, where circulating APOA1 serves as a key surrogate marker for HDL functionality and reverse cholesterol transport. The assay accepts serum, plasma, tissue homogenates, cell lysates and cell culture supernatants, allowing investigators to profile hepatic APOA1 expression in rodent models as well as systemic lipoprotein dynamics. Quantitative readouts are obtained through an enzymatic HRP-TMB reaction read on standard microplate readers, and the kit is supplied with pre-coated plates, biotinylated detection antibody, and streptavidin-HRP conjugate sufficient for 96 determinations. Typical intra-assay and inter-assay precision for the kit is under 10% CV, with a detection range of approximately 1.56 to 100 ng/mL and a sensitivity below 0.5 ng/mL using 50 µL of sample. Researchers commonly apply this kit to studies of atherosclerosis, diabetes-associated dyslipidemia, and hepatocyte lipid handling, where serial sampling from rat cohorts demands a robust, high-throughput immunoassay. Unopened reagents should be stored refrigerated at 4 °C until first use; reconstituted standards remain stable for short-term storage at -20 °C, and repeated freeze-thaw cycles should be avoided to preserve antibody binding activity.