Rat Apelin 13 (AP13) ELISA Kit

Measuring apelin-13, the most potent C-terminal fragment of preproapelin and endogenous ligand for the APJ (APLNR) G-protein-coupled receptor, this competitive ELISA from Assay Genie is configured for 96 determinations using rat-derived specimens.

The plate-based, competitive format relies on endogenous peptide in the experimental specimen competing with peptide pre-coated onto the microplate for binding sites on a labelled anti-apelin-13 antibody. Colorimetric development yields an inverse signal in which higher absorbance corresponds to lower analyte concentration, a configuration matched to small endogenous peptides such as the 13-residue apelin-13. The kit supplies peptide standard, capture and detection reagents, wash buffer, substrate and stop solution as break-apart vials, allowing single-use flexibility across runs.

Routinely deployed in studies of vascular tone, cardiac contractility, angiogenesis, fluid homeostasis and insulin signalling, the assay supports longitudinal profiling of the apelin/APJ axis in rat models of hypertension, heart failure, diabetes and obesity.