Rat Annexin A5/ANXA5 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the quantitative measurement of Annexin A5 (ANXA5) in rat samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit uses a colorimetric detection method with a double antibody sandwich format. Annexin A5 is described as a key regulator of cell membrane structure and function, with roles in apoptosis and blood coagulation, and its dysregulation has been linked to inflammatory disorders and cancer, supporting its use as a biomarker for disease progression and a potential therapeutic target.

Performance characteristics include a sensitivity of 0.188 ng/mL and an assay range of 0.313-20 ng/mL. Linearity has been evaluated across serial dilutions: serum (n=5) showed 87-102% at 1:2, 86-101% at 1:4, and 83-100% at 1:8; EDTA plasma (n=5) showed 86-98%, 82-93%, and 81-99%; heparin plasma (n=5) showed 86-105%, 82-101%, and 82-89%. Recovery was demonstrated in serum (89-102%, average 93%), EDTA plasma (85-101%, average 93%), and heparin plasma (86-105%, average 92%).

The kit is intended for storage at 2-8°C for 12 months and is associated with cardiovascular research. The associated UniProt identifier is P14668, with documented synonyms including Annexin V, Annexin-5, Lipocortin V, Endonexin II, Calphobindin I (CBP-I), Anchorin CII, Vascular anticoagulant-alpha (VAC-alpha), Placental anticoagulant protein I (PAP-I), Placental anticoagulant protein 4 (PP4), and Thromboplastin inhibitor.