Rat Annexin A4 (Anxa4) ELISA Kit

A sandwich ELISA kit from Assay Genie designed for the quantitative measurement of Annexin A4 (Anxa4) in rat samples. The kit is reactive with rat and recognises natural and recombinant rat Annexin A4. Synonyms listed for the target include 36 kDa zymogen granule membrane-associated protein, Annexin IV, Annexin-4, Lipocortin IV, ZAP36 and Anx4. The assay uses a colorimetric detection method and is catalogued for cardiovascular research. It is for research use only.

Reported performance characteristics include a detection range of 0.156-10ng/mL and a sensitivity of 0.08ng/mL. Intra-assay CV is 4.3% and inter-assay CV is 8.8%. Recovery averages 99% for serum (range 93-105%) and 101% for plasma (range 95-107%). Linearity across dilutions of 1:2 to 1:16 is reported as 107-118%, 87-95%, 105-114% and 102-115% for serum; 99-109%, 111-120%, 105-115% and 106-116% for EDTA plasma; and 108-117%, 109-117%, 103-113% and 99-109% for heparin plasma (N=5 per matrix).

Validated sample types include serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Annexin A4 is described as a calcium/phospholipid-binding protein that promotes membrane fusion and is involved in exocytosis. The UniProt entry for the target protein is P55260.