Rat Angiotensin II receptor type 1/AT1R ELISA Kit

A high-quality sandwich ELISA kit from Assay Genie for the quantitative detection of Angiotensin II receptor type 1 (AT1R) in rat samples. The assay uses a double-antibody sandwich format and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types.

Sensitivity is 46.875 pg/mL with a working range of 78.125–5000 pg/mL. Reported linearity for serum, EDTA plasma and heparin plasma across 1:2, 1:4 and 1:8 dilutions spans 81–105%. Recovery performance is consistent across matrices, averaging 91% in serum, 96% in EDTA plasma and 94% in heparin plasma. Reagents are stored at 2–8°C and remain stable for 12 months. The protocol involves plate equilibration, primary incubation at 37°C, biotinylated detection antibody binding, HRP-streptavidin conjugation, TMB colour development and absorbance reading at 450 nm.

Angiotensin II receptor type 1 is a central component of the renin-angiotensin system, regulating blood pressure and fluid balance, with dysregulation implicated in cardiovascular, renal and metabolic disease research.