Rat Angiopoietin Like Protein 6 (ANGPTL6) ELISA Kit

Quantitative ELISA measurement of rat ANGPTL6 is intended for research use only, supporting investigations into metabolic disease, vascular biology, and cutaneous wound repair, where altered serum concentrations of this hepatokine have been documented in rodent models of obesity, insulin resistance, and type 2 diabetes. Circulating ANGPTL6 also functions as an adipokine linked to systemic energy homeostasis, which is why longitudinal profiling in rat cohorts has become a useful approach for studying diet-induced metabolic syndrome and related cardiovascular complications.

This Assay Genie kit is supplied as a 96-assay configuration containing a pre-coated microplate, lyophilized recombinant ANGPTL6 standard, matched detection antibody, enzyme conjugate, wash concentrate, substrate, and stop reagent, with reagents sufficient to develop reproducible standard curves across a chromogenic read-out compatible with standard laboratory plate readers. The protocol is validated for serum samples, plasma preparations, and tissue-derived homogenates collected from rat specimens. Unopened components should be refrigerated, and reconstituted standards are best aliquoted and frozen to limit repeated freeze-thaw cycles across multi-day runs.

Typical workflows include tracking circulating ANGPTL6 in diet-induced obesity cohorts, wound-healing time-course studies, angiogenesis assays, and ex vivo profiling of conditioned media from primary rat hepatocytes or adipocytes. Comparative work between treatment and control groups, including pharmacological interventions targeting the angiopoietin-like protein family, is also supported by the kit's broad sample compatibility.