Rat Ang1-9 (Angiotensin 1-9) ELISA Kit

Assay Genie provides this ELISA kit for the quantitative measurement of Angiotensin 1-9 in rat-derived samples, supporting research into the renin-angiotensin system and its role in cardiovascular regulation. The assay is configured as a competitive ELISA with antigen-coated wells and uses a colorimetric readout, with absorbance read at 450 nm following TMB substrate development.

Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with reactivity confirmed in rat. Sensitivity is reported at 3.75 pg/mL and the working range is 6.25-400 pg/mL. Linearity has been assessed across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions, with recovery ranges also reported for these three matrices. The kit is supplied sealed for storage at 2-8°C and should not be cryopreserved.

The protocol calls for equilibrating TMB substrate for 30 minutes at room temperature, adding 50 µL of standard or sample together with 50 µL of biotin-labelled antibody per well, and incubating at 37°C for 45 minutes. Plates are washed five times with one-minute soak intervals, colour is developed in the dark for 10-20 minutes, and the reaction is stopped before absorbance is measured. Sample-specific centrifugation conditions are described for serum, plasma, cell culture supernatant, cell lysate, and tissue homogenate preparations.