Rat Amyloid Beta 40/AB 1-40 ELISA Kit

Assay Genie's Rat Amyloid Beta 40 (AB 1-40) ELISA Kit is a sandwich ELISA using a double antibody format, designed for the accurate detection of amyloid beta 40 levels in rat samples. The kit is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, cerebrospinal fluid (CSF), and other liquid samples, employing a colorimetric detection method with absorbance measured at 450 nm.

Reported sensitivity is 46.875 pg/mL with an assay range of 78.125-5000 pg/mL. The kit should be stored at 2-8°C for 12 months. Linearity values are: serum (n=5) 92-100% at 1:2, 88-105% at 1:4, 89-105% at 1:8; EDTA plasma (n=5) 86-99%, 82-90%, 87-96%; heparin plasma (n=5) 84-100%, 87-98%, 80-104%. Recovery ranges and averages are: serum (n=5) 92-100% / 96%; EDTA plasma (n=5) 88-104% / 93%; heparin plasma (n=5) 88-104% / 97%. The associated Uniprot ID is P08592 and the protein is also known as ABeta40, Abeta 40, Beta-amyloid protein 40, and ABeta 1-40.

The assay procedure includes reagent and plate preparation, primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation at 37°C for 60 minutes, HRP-Streptavidin (SABC) binding for 30 minutes, TMB color development in the dark for 10-20 minutes, and stop reaction followed by absorbance reading. Amyloid beta 40 is a key protein implicated in Alzheimer's disease and other neurodegenerative disorders, making it a critical biomarker for studying disease pathology and developing potential treatments.