Rat AMY2/Amylase Alpha 2 ELISA Kit

Designed to quantify Amylase Alpha 2 in rat-derived samples, this double-antibody Sandwich ELISA Kit is supplied by Assay Genie. A colorimetric readout is produced with a TMB substrate measured at 450 nm, and the kit is intended for metabolism research where amylase dysregulation has been linked to diabetes and pancreatic disorders. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Unopened kits are stored at 2–8°C for 12 months.

Reported analytical performance includes a sensitivity of 0.938 ng/mL and an assay range of 1.563–100 ng/mL. Recovery averages 97% in serum (range 87–104%), 98% in EDTA plasma (range 90–103%) and 98% in heparin plasma (range 91–105%). Dilutional linearity at 1:2, 1:4 and 1:8 lies between 91–104%, 84–100% and 86–100% for serum, 85–102%, 82–101% and 87–100% for EDTA plasma, and 89–105%, 82–97% and 81–94% for heparin plasma.

Recognised synonyms for the target protein include Amy2, PA, AMY2, Amy2a, Pancreatic alpha-amylase and 1,4-alpha-D-glucan glucanohydrolase (UniProt P00689).