Rat Aminolevulinate Delta Synthase 1 (ALAS1) ELISA Kit

Aminolevulinate Delta Synthase 1 (ALAS1) is the first and rate-limiting enzyme of the heme biosynthesis pathway, catalyzing the condensation of glycine with succinyl-CoA to form 5-aminolevulinic acid in the mitochondrial matrix. This Assay Genie ELISA kit is configured for quantitative measurement of rat ALAS1 in tissue homogenates, supporting research into hepatic heme metabolism, erythropoiesis regulation, and altered porphyrin synthesis. The kit provides pre-coated plate wells, rat ALAS1 standards, detection reagents, and the wash and substrate buffers needed to complete the immunoassay workflow in a 96-assay plate format. Researchers studying mitochondrial enzyme expression, liver injury models, or iron-homeostasis pathways can use the assay to compare ALAS1 protein levels across treated versus control rat samples. Reagents are intended for research use only, and the protocol is compatible with standard microplate readers in laboratories profiling heme-biosynthesis activity.