Rat Alpha-N-acetylgalactosaminidase/NAGA ELISA Kit

Assay Genie offers a sandwich ELISA kit designed for the quantitative detection of rat Alpha-N-acetylgalactosaminidase (NAGA), a lysosomal enzyme involved in glycoprotein degradation. The assay uses a colorimetric detection method with a double-antibody sandwich format, supporting research into NAGA activity, lysosomal function, and the role of NAGA dysregulation in disease.

Reactive with rat samples, the kit is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported performance characteristics include a sensitivity of 0.188 ng/mL and a detection range of 0.313-20 ng/mL. Linearity testing in serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions returned values between 80-105%, and spike recovery ranged from 85-105% across the same sample types. Reagents are intended to be stored at 2-8°C for 12 months.

The kit is supplied with components including a pre-coated ELISA microplate, lyophilized standard, biotin-labeled detection antibody, HRP-Streptavidin conjugate (SABC), TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, stop solution, wash buffer, plate sealer, and a technical manual. The associated UniProt identifier for rat NAGA is Q66H12.