Rat alpha 1-Microglobulin/alpha 1-MG ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA, double antibody kit is designed for the quantitative detection of alpha-1-microglobulin in rat samples. The assay uses a colorimetric detection method and is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples.

Key performance specifications include a sensitivity of 3.75 ng/mL and a measurable range of 6.25-400 ng/mL. Spiking recovery is reported at 85-99% (average 91%) for serum, 87-102% (average 95%) for EDTA plasma, and 89-104% (average 97%) for heparin plasma (n=5 each). Reagents should be stored at 2-8°C for 12 months.

Alpha-1-microglobulin is implicated in renal function, oxidative stress, and inflammation, with abnormal levels linked to kidney disease, urinary tract infections, and related conditions, making it a relevant biomarker for biomedical research. The workflow uses a pre-coated microplate with sample and standard incubation, biotin-labeled detection antibody binding, HRP-streptavidin incubation, TMB color development, and absorbance reading at 450 nm. Reagents provided include lyophilized standard, biotin-labeled antibody, HRP-streptavidin conjugate, TMB substrate, sample and antibody dilution buffers, SABC dilution buffer, wash buffer, stop solution, plate sealer, and a technical manual.