Rat ALOX5AP/FLAP ELISA Kit

This Sandwich ELISA Kit from Assay Genie is designed for the accurate quantification of ALOX5AP (FLAP) levels in rat samples. ALOX5AP, also known as FLAP, plays a key role in the arachidonic acid pathway and leukotriene biosynthesis, and is involved in inflammatory responses with implications in diseases such as asthma, atherosclerosis, and inflammatory disorders. The kit supports research in immunology, pharmacology, and disease pathology, and is relevant to the metabolism research area.

The assay uses a colorimetric Sandwich ELISA, Double Antibody detection method and is reactive with rat samples. It is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Storage is at 2-8°C for 12 months.

Performance specifications include a sensitivity of 9.375 pg/mL and an assay range of 15.625-1000 pg/mL. Linearity at the 1:2 dilution is 87-103% for serum, 84-100% for EDTA plasma, and 80-95% for heparin plasma. Recovery averages 94% for serum, 92% for EDTA plasma, and 96% for heparin plasma.