Rat Alkaline Phosphatase, Intestinal (ALPI) ELISA Kit

Built around the intestinal brush-border enzyme alkaline phosphatase (ALPI), this quantitative immunoassay kit from Assay Genie measures ALPI levels in rat research specimens. ALPI is a glycosylphosphatidylinositol-anchored enterocyte enzyme that contributes to lipopolysaccharide detoxification at the gut mucosal surface, modulates luminal pH at the brush border, and supports long-chain fatty acid absorption. Altered ALPI expression has been documented in animal models of colitis, high-fat-diet feeding, necrotizing enterocolitis, and antibiotic-induced dysbiosis, making this marker useful for nutrition, gastroenterology, and microbiome research programs. Each kit ships as a complete microplate package with pre-coated wells and the reagents required to run the immunoassay workflow end-to-end. The assay processes rat specimens, supporting serum samples, plasma draws, and tissue homogenate preparations. Downstream experiments frequently pair ALPI quantification with western blot confirmation, immunohistochemistry on intestinal sections, or cell culture work on enterocyte models. Storage requires refrigeration of kit components between uses, and the package is intended for research use only.