Rat Aldolase B, Fructose Bisphosphate (ALDOB) ELISA Kit

Quantitative measurement of rat Aldolase B, Fructose Bisphosphate (ALDOB) is enabled by this enzyme immunoassay kit from Assay Genie. Aldolase B is a key glycolytic and gluconeogenic enzyme that catalyzes the reversible cleavage of fructose-1,6-bisphosphate into glyceraldehyde-3-phosphate and dihydroxyacetone phosphate, and it also processes fructose-1-phosphate in hepatic tissue. Mutations affecting this enzyme cause hereditary fructose intolerance, and altered hepatic aldolase B expression has been documented in rodent models of liver injury, metabolic syndrome, and dietary fructose exposure.

Supplied in a 96-assay configuration, the kit contains a pre-coated microplate, assay buffers, detection reagents, and standards required to generate a standard curve and quantify unknown samples. Researchers in metabolic disease, hepatology, and nutritional biochemistry can incorporate the kit into studies comparing aldolase B antigen levels between treatment groups in rat model systems. The procedure follows a stepwise incubation and wash protocol compatible with standard laboratory plate readers.

For reliable performance, store kit components refrigerated, protect from light, and allow reagents to reach room temperature before use. Each kit is configured for in vitro research use only and should be handled by personnel trained in enzyme immunoassay workflows.