Rat Agouti Related Protein (AGRP) ELISA Kit

Centrally produced by neurons of the hypothalamic arcuate nucleus, Agouti-Related Protein (AGRP) is an orexigenic neuropeptide that antagonizes melanocortin-3 (MC3R) and melanocortin-4 (MC4R) receptors to drive feeding responses during negative energy balance. AGRP is commonly co-expressed with neuropeptide Y in a distinct population of mediobasal hypothalamic neurons, and its transcript and circulating levels rise sharply during fasting as part of the adaptive counter-regulatory response to calorie restriction.

This rat ELISA kit from Assay Genie is supplied as a 96-assay reagent set for quantitative measurement of AGRP in blood-derived and tissue sample matrices, with all microplates, buffers, and detection components required to run a full plate. The assay format uses a research-grade antibody pair directed against rat AGRP and a reporter system producing absorbance readings on a standard microplate reader. Intended for in vitro research applications, the kit supports studies of appetite regulation, hypothalamic neuropeptide signaling, obesity and metabolic disease models, energy balance, and pharmacological evaluation of melanocortin pathway modulators.

Sample handling protocols for AGRP measurement in published literature typically describe blood-derived preparations and hypothalamic tissue homogenates processed with protease inhibitors, snap-frozen, and stored until batched analysis. Reconstituted kit reagents maintain performance over several weeks at refrigerated storage temperatures when handled per the supplied instructions; long-term storage of unopened kit components is at -20°C or lower, with freeze-thaw cycles minimized. A full protocol booklet and plate layout template accompany the kit, including recommended dilution factors for individual specimen types and procedural notes for the supplied standards.

The kit is for research use only and is not intended for diagnostic procedures. Each manufactured lot is qualified against recombinant rat AGRP reference material to confirm consistent inter-plate performance and reproducible quantification across runs.