Rat Adrenergic Receptor Beta Kinase 1/ADRBETAK1 ELISA Kit

Designed for quantitative measurement in rat samples, this Sandwich ELISA kit from Assay Genie targets Adrenergic Receptor Beta Kinase 1 (ADRBETAK1), a key enzyme involved in the regulation of adrenergic receptor signaling pathways, with a role in the modulation of cardiac function and vascular tone.

Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Linearity in serum, EDTA plasma and heparin plasma at 1:2, 1:4 and 1:8 dilutions spanned 81-102%, while recovery averaged 95% for serum, 96% for EDTA plasma and 94% for heparin plasma. The kit should be stored at 2-8°C for 12 months.

Built on a double-antibody sandwich format with colorimetric detection, the workflow uses a biotin-labelled detection antibody, HRP-Streptavidin binding, and TMB substrate development, with absorbance read at 450 nm.