Rat Adrenergic Receptor Beta 3 (ADRb3) ELISA Kit

The Adrenergic Receptor Beta 3 (ADRB3) is a G-protein-coupled receptor that mediates catecholamine-driven lipolysis in adipose tissue and smooth muscle relaxation in the bladder detrusor and gut. Quantitative tracking of receptor abundance supports research into brown adipose thermogenesis, metabolic syndrome models, obesity interventions, and beta-agonist pharmacology.

This two-antibody immunoassay from Assay Genie is calibrated for rat tissue homogenates and uses an absorbance-based microplate endpoint to report ADRB3 protein levels. The 96 assay kit contains a pre-coated microplate, lyophilized reference standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent, providing all components needed to run a complete standard curve plus unknowns in replicates. The format accommodates both small pilot studies comparing few treatment groups and larger tissue panels drawn from individual animals.

Unopened kit components are shipped refrigerated and should be stored protected from light until first use. Reconstituted standards and working detection antibody should be aliquoted and frozen at -20 degrees C to preserve activity across multiple freeze-thaw cycles. The kit is designated for research use only in laboratory settings and is not intended for clinical diagnostic work.