Rat ADORA2b (Adenosine A2b Receptor) ELISA Kit (RTES01125)

Assay Genie's Rat ADORA2B Adenosine A2B Receptor ELISA Kit supports quantitative measurement of Adora2b, a seven-transmembrane G-protein coupled receptor that binds extracellular adenosine and modulates cAMP signaling, vascular smooth muscle relaxation, mast cell activation, and adaptive responses to hypoxia. Researchers in cardiovascular, pulmonary, and renal physiology use this microplate-based assay to compare receptor expression across treated and untreated cohorts, or to track modulation in cell culture and in vitro inflammation models. The kit supplies a pre-coated plate, biotinylated detection antibody, HRP conjugate, wash buffer, and recombinant rat Adora2b standards for generating a standard curve. Reconstituted reagents should be stored at -20°C in single-use aliquots to limit freeze-thaw cycles, while the unopened kit is stable refrigerated for 6 months. Each run requires a 50 µL sample volume per well, and the protocol is compatible with serum samples, plasma samples, and tissue homogenate matrices commonly used in cardiovascular research.