Rat Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

Quantify Adiponectin Receptor 1 (ADIPOR1) in rat-derived samples with this in vitro ELISA kit from Assay Genie, configured for research use only in metabolic and endocrine studies. ADIPOR1 is a seven-transmembrane receptor that binds adiponectin and couples to AMPK and PPAR-alpha pathways, driving fatty-acid oxidation and glucose uptake in skeletal muscle and liver; measuring its abundance supports investigations into insulin resistance, type 2 diabetes, obesity, and non-alcoholic fatty liver disease in rodent models.

The kit ships as a complete package containing a pre-coated microplate, detection antibody, recombinant standards, and the buffers required to run the assay. Researchers can apply it to samples such as serum, tissue homogenate, or cell lysate preparations, reading the colourimetric signal on a standard plate reader. Reconstituted reagents should be divided into aliquots to limit freeze-thaw cycles; the kit is shipped refrigerated, components should be stored refrigerated when not in use, and light-sensitive reagents should be protected from light.

Each pack supports 96 assays, making it practical for medium-throughput screening of rat cohorts. The procedure relies on a matched antibody pair, with a capture antibody immobilised on the plate and a conjugated detection antibody producing colour proportional to ADIPOR1 concentration in each well. Pair this kit with downstream western blot or PCR confirmation when characterising receptor regulation across treatment groups.