Rat ADAMTS5 (A Disintegrin And Metalloproteinase With Thrombospondin 5) ELISA Kit (RTFI01458)

Quantitative measurement of rat ADAMTS5, a zinc-dependent aggrecanase implicated in cartilage matrix catabolism, is supported by this ELISA kit from Assay Genie. ADAMTS5 (A Disintegrin And Metalloproteinase with Thrombospondin motifs 5) cleaves aggrecan at the Glu373–Ala374 bond and is the dominant aggrecan-degrading activity targeted in rodent models of osteoarthritis and rheumatoid arthritis; circulating serum levels of this enzyme therefore serve as a biomarker of ongoing extracellular matrix turnover in articular cartilage.

Supplied as a 96 Assays pack for in vitro research use, the assay is calibrated for rat reactivity and provides the reagents required for a colorimetric procedure, including a pre-coated microplate ready for sample loading. It is well matched to preclinical arthritis studies, joint tissue biology experiments, and pharmacology screens evaluating disease-modifying osteoarthritis compounds or matrix-protective interventions, and may also support cardiovascular research where ADAMTS5 has been linked to vascular remodeling and atherosclerotic plaque instability.