Rat Actin Beta (ACTb) ELISA Kit

Beta-actin, a 42 kDa cytoskeletal housekeeping protein encoded by the Actb gene, is the most widely used loading-control target in rat cell biology, supporting western blot and RT-PCR normalization across many published experiments. The corresponding rat ACTb enzyme-linked immunosorbent assay from Assay Genie provides a plate-based quantitative read on total beta-actin levels, giving researchers an orthogonal antibody-based output that complements standard gel-based normalization workflows.

Sample inputs accepted include tissue homogenates, cell lysates, and cell culture supernatants, supporting applications such as cytoskeletal studies, rat model validation, and confirmation of expression-level controls in cell biology research. Each kit ships as 96 assays in a sealed box format, with reagents intended for research use only. Following the workflow, signal is read on a standard laboratory plate reader. Storage of unopened components around 4 °C under refrigerated conditions is recommended; reconstituted standards and detection reagent should be aliquoted to limit freeze-thaw cycles and held per the lot-specific insert.