Rat AANAT/Serotonin N-acetyltransferase ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA kit is designed for the quantitative detection of rat Serotonin N-acetyltransferase (AANAT) using a double-antibody format and colorimetric readout. The assay is intended for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with a reported sensitivity of 37.5 pg/mL and a working range of 62.5-4000 pg/mL.

Serotonin N-acetyltransferase is a key enzyme in melatonin biosynthesis and is therefore central to studies of circadian rhythm and sleep-wake cycle regulation; its dysregulation has been associated with various neurological and psychiatric conditions, making it a useful biomarker in cell biology research. According to the manufacturer's performance data, the kit shows overall linearity of 86-103% (serum), 86-100% (EDTA plasma) and 80-100% (heparin plasma) across serial dilutions, with average spike-recovery values of 96% in serum, 96% in EDTA plasma and 97% in heparin plasma.

The kit should be stored at 2-8°C and remains stable for 12 months.