Rat 6-phosphofructokinase type C/PFKP ELISA Kit

Phosphofructokinase type C (PFKP) is a key enzyme in glycolysis, playing a crucial role in energy production and metabolism. Dysregulation of PFKP has been associated with various metabolic disorders and diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions. Assay Genie provides a highly sensitive and specific sandwich ELISA built on a double-antibody configuration for the accurate detection of PFKP levels in rat serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Detection is colorimetric.

The assay delivers a sensitivity of 0.188 ng/mL across a working range of 0.313-20 ng/mL. Linearity was verified at 1:2, 1:4 and 1:8 dilutions: serum (n=5) showed ranges of 87-101%, 85-105% and 85-92%; EDTA plasma (n=5) showed 92-100%, 85-99% and 82-98%; and heparin plasma (n=5) showed 82-96%, 84-98% and 84-95%. Recovery was confirmed across matrices, with serum (n=5) averaging 100% (89-105% range), EDTA plasma (n=5) averaging 94% (89-98% range), and heparin plasma (n=5) averaging 95% (87-103% range). The kit is stored at 2-8°C for 12 months.

Overall, this Assay Genie PFKP ELISA kit offers researchers a reliable and reproducible tool for investigating the role of PFKP in metabolism and its implications in disease pathology.