Rat 5'-nucleotidase/5-NT ELISA Kit

From Assay Genie, this ELISA kit enables detection and quantification of 5'-nucleotidase (5'-NT) in rat samples, including serum, plasma, cell culture supernatants, cell or tissue lysates, and other liquid samples. 5'-NT is an enzyme involved in nucleotide metabolism and has been implicated in liver disease, kidney dysfunction, and inflammatory disorders.

The kit offers a sensitivity of 1.875 pg/mL with an assay range of 3.125-200 pg/mL. Linearity in serum, EDTA plasma, and heparin plasma (n=5) ranges from 82-104% across 1:2, 1:4, and 1:8 dilutions. Average recovery values are 92% for serum, 97% for EDTA plasma, and 95% for heparin plasma. Reagents are stored at 2-8°C for 12 months.

Included components cover the full assay workflow: a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody, HRP-streptavidin conjugate, TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer, plate sealer, and a technical manual. The target protein is associated with Uniprot P21588 and the synonym Nt5e.