Rat 3MH (3-methylhistidine) ELISA Kit

Designed for the precise measurement of 3-methylhistidine levels in rat samples, this competitive ELISA from Assay Genie is a highly sensitive and specific assay suitable for a range of research applications. 3-methylhistidine is a biomarker that reflects muscle protein turnover and is commonly used in studies related to muscle catabolism, exercise physiology, and nutritional assessment. Monitoring 3-methylhistidine levels can provide valuable insights into muscle health and metabolism, supporting research in muscle physiology, nutrition, and muscle-related disorders and interventions.

The assay is coated with antigen and uses a colorimetric detection method, with a sensitivity of 3.75 nmol/mL and a detection range of 6.25-400 nmol/mL. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit should be stored sealed at 2-8°C and should not be cryopreserved.

Reported linearity across 1:2, 1:4, and 1:8 dilutions: serum (n=10) 86-102%, 93-105%, 92-102%; EDTA plasma (n=10) 86-98%, 93-101%, 92-99%; heparin plasma (n=10) 86-100%, 85-101%, 82-99%. Reported recovery: serum (n=10) 85-102%, average 96%; EDTA plasma (n=10) 85-98%, average 95%; heparin plasma (n=10) 88-103%, average 98%.