Rat 12-LO (Arachidonate 12-Lipoxygenase) ELISA Kit (RTES00834)

For quantitative determination of arachidonate 12-lipoxygenase (12-LO) in rat samples, this ELISA kit from Assay Genie provides a useful tool in lipid mediator research. 12-LO is a non-heme iron-containing dioxygenase that converts arachidonic acid into 12-hydroperoxyeicosatetraenoic acid (12-HPETE), which is subsequently reduced to 12-hydroxyeicosatetraenoic acid (12-HETE), a bioactive lipid implicated in platelet aggregation, vascular inflammation, and certain oncogenic pathways. The kit is configured for 96 assays and is intended for in vitro research use only. Researchers studying the lipoxygenase pathway in rat models of cardiovascular disease, hepatic steatosis, diabetes mellitus, or tumor biology may use this assay to monitor enzyme levels across experimental conditions. The protocol is suitable for laboratory personnel familiar with standard ELISA workflows and includes all reagents necessary to run the assay plate to completion.