RAD52 (Phospho-Tyr104)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this cell-based ELISA kit is designed for the precise detection of RAD52 phosphorylation at tyrosine 104 in cell lysates and culture supernatants. It detects both the phosphorylation state and expression profile of the RAD52 protein in cultured cells, supporting measurement of the relative amounts of phosphorylated RAD52 as well as screening for the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators on RAD52 phosphorylation.

The assay is lysate-free, high-throughput, and uses an indirect ELISA format. Phospho-specific primary antibodies capture RAD52 (Phospho-Tyr104), and HRP-conjugated secondary antibodies bind the Fc region of the primary antibody to catalyze a colorimetric reaction measured at 450 nm. A monoclonal antibody specific for human GAPDH is included as an internal positive control for normalization, and Crystal Violet whole-cell staining can be used to determine cell density. The kit is reactive with human and mouse samples and is intended for adherent cells, with a dynamic range of > 5000 cells.

RAD52 is a key protein involved in DNA double-strand break repair and homologous recombination, contributing to genomic stability. Phosphorylation at tyrosine 104 is known to modulate RAD52's function in DNA repair, making this site a relevant biomarker for studies of DNA damage response mechanisms and for potential therapeutic research in cancer and other genetic disorders.