Rabphilin 3A (Phospho-Ser237)Colorimetric Cell-Based ELISA Kit

From Assay Genie, this colorimetric cell-based ELISA kit enables qualitative detection of phosphorylated Rabphilin-3A at Ser237 in cultured cells using an indirect ELISA format. Phospho-specific primary antibodies capture the target, and HRP-conjugated secondary antibodies catalyze a colorimetric reaction read at 450 nm. A monoclonal anti-GAPDH antibody is included as an internal positive control for normalization, while an optional Crystal Violet whole-cell staining step allows cell density to be factored into the analysis.

Reactive with human, mouse, and rat samples and intended for adherent cells, the kit supports a lysate-free, high-throughput workflow suitable for screening the effects of treatments, inhibitors, or activators on Rabphilin-3A phosphorylation, with a dynamic range of > 5000 Cells. Rabphilin-3A is a regulator of membrane trafficking and neurotransmitter release that plays a key role in synaptic transmission and neuronal function, and dysregulation of its phosphorylation has been implicated in neurological disorders including Alzheimer's disease and Parkinson's disease, making this tool relevant to research on intracellular signaling, synaptic plasticity, and neurodegeneration.